Tris-HCl Buffer, pH 7.2 [1M] - 1000 ml
Tris-HCl buffer has many applications in molecular biology work, including use in nucleic acid purification.
Tris-HCl Buffer, pH 7.2 [1M] - 1000 ml
Artigo n.º: 94951804

Tris-HCl Buffer, pH 7.2 [1M] - 1000 ml

Artigo n.º: 94951804

STD 2297515

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Tris-HCl buffer has many applications in molecular biology work, including use in nucleic acid purification.
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O que se Destaca

Consistent pH
Offers a stable pH of 7.2, essential for biological experiments and applications, ensuring accurate and reliable results for precise experimental conditions.
High Purity
Made with high-quality reagents that minimize contamination, making it suitable for sensitive assays and research, enhancing the reliability of experimental results.
Convenient Size
Available in a 1000 ml volume, providing ample buffer for extensive laboratory needs, reducing the frequency of repurchase and ensuring long-term usability.

Detalhes do produto

Shop Tris-HCl Buffer, pH 7.2 [1M] - 1000 ml online at a best price in São Tomé and Príncipe. B09V5MRJGF
Item Weight2.2 lbs (1 kg)

Quem Deverá Comprar?

Suitable For
  • Biochemistry Labs

    Ideal for biochemistry labs conducting experiments requiring stable pH conditions for enzyme reactions and protein studies.

  • Molecular Biology

    Suitable for molecular biology applications, particularly in DNA and RNA extraction protocols needing consistent buffering.

  • Educational Use

    Great for educational institutions conducting practicals to teach students about buffer solutions and biochemical reactions.

Not Suitable For
  • Non-scientific Use

    Not suitable for individuals or applications outside of scientific research, lacking practical non-laboratory utility.

  • Extreme pH Needs

    Unsuitable for experiments requiring pH levels significantly above or below 7.2, limiting its versatility.

  • Storage Limitations

    Not recommended for long-term storage; may degrade over time if not stored under appropriate conditions.

DESCRIÇÃO DO PRODUTO

Tris-HCl Buffer, pH 7.2 [1M] - 1000 ml

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Perguntas e Respostas dos Clientes

  • pergunta: What is Tris-HCl Buffer and what is its purpose?

    Resposta: Tris-HCl Buffer is a widely used buffering agent that helps maintain a stable pH in biological and chemical experiments. It is particularly effective around a neutral pH of 7.2. The buffer prevents sudden changes in pH, which is critical in experiments involving proteins, nucleic acids, and enzymatic reactions, where even minor pH fluctuations can impact results.
  • pergunta: How do I prepare Tris-HCl Buffer at pH 7.2?

    Resposta: To prepare Tris-HCl Buffer at pH 7.2, you will need Tris base and hydrochloric acid (HCl). Start by dissolving the specific amount of Tris base in distilled water and then slowly add HCl while continuously monitoring the pH until you reach the desired level of 7.2. This method allows precise control over the buffer composition, ensuring optimal performance in your experiments.
  • pergunta: What applications are suitable for Tris-HCl Buffer, pH 7.2?

    Resposta: Tris-HCl Buffer at pH 7.2 is primarily used in molecular biology applications such as DNA and RNA extraction, gel electrophoresis, and enzyme assays. Its ability to maintain a stable pH makes it a favorite choice in protocols involving sensitive biochemical processes. Additionally, it's commonly utilized in cell culture mediums to ensure optimal growth conditions.
  • pergunta: Can I use Tris-HCl Buffer for protein stability studies?

    Resposta: Yes, Tris-HCl Buffer is often utilized in protein stability studies. Its neutral pH environment helps preserve the structure and functionality of proteins, which is essential when investigating protein interactions or enzyme activity. Using this buffer ensures that the proteins remain in an optimal pH range throughout the experiment, thus providing reliable and consistent data.
  • pergunta: What is the concentration of Tris in Tris-HCl Buffer, pH 7.2?

    Resposta: In Tris-HCl Buffer, pH 7.2, the concentration of Tris is typically 1M. This indicates the amount of Tris present in the solution, and it is crucial for maintaining the desired buffering capacity. A 1M concentration ensures that even with the addition of acids or bases during experiments, the buffer can effectively stabilize the pH of the solution.
  • pergunta: Is Tris-HCl Buffer suitable for use in immunoassays?

    Resposta: Yes, Tris-HCl Buffer, pH 7.2, is suitable for use in immunoassays. Its buffering capacity ensures stable pH levels, which is essential for the performance and specificity of antibodies during assay procedures. This stability allows for consistent results, making Tris-HCl Buffer a preferred choice for various immunological applications.
  • pergunta: How should I store Tris-HCl Buffer once opened?

    Resposta: After opening, Tris-HCl Buffer should be stored in a cool, dark place, preferably in a tightly closed container to prevent contamination. Keeping the buffer protected from light and temperature fluctuations will help maintain its quality and effectiveness for future use. Proper storage is key to ensuring the buffer retains its pH stability and overall properties.
  • pergunta: Can Tris-HCl Buffer be used in cell culture?

    Resposta: Indeed, Tris-HCl Buffer is commonly utilized in cell culture. It helps maintain the right pH for the growth of various cell lines, providing an optimal environment for cellular functions. This buffer is particularly beneficial in studies involving cellular responses where pH plays a critical role in biochemical pathways and interactions.
  • pergunta: What is the shelf life of Tris-HCl Buffer?

    Resposta: The shelf life of Tris-HCl Buffer is generally quite long if stored properly, typically lasting several years. However, it is important to monitor the pH and overall clarity of the solution over time. If any changes are observed in its physical or chemical properties, it may be best to prepare a fresh solution to ensure experimental reliability.
  • pergunta: Where can I buy Tris-HCl Buffer, pH 7.2 [1M] - 1000 ml?

    Resposta: You can purchase Tris-HCl Buffer, pH 7.2 [1M] - 1000 ml at Ubuy. They offer a wide variety of laboratory supplies, including biochemicals and reagents like Tris buffers, ensuring you can find the right products for your research needs.

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  • Limitações: para produtos expedidos internacionalmente, tenha em atenção que qualquer garantia do fabricante pode não ser válida; opções de serviço do fabricante podem não estar disponíveis; manuais de produtos, instruções e avisos de segurança podem não estar nas línguas do país de destino; os produtos (e materiais que o acompanham) pode não ter sido concebido em conformidade com as normas, especificações e requisitos de rotulagem do país de destino; e os produtos podem não estar em conformidade com a voltagem e outras normas elétricas do país de destino (requerendo assim o uso de um adaptador ou conversor caso seja apropriado). O destinatário é responsável por garantir que o produto pode ser legalmente importado para o país de destino. Quando encomenda à Ubuy ou aos seus afiliados, o destinatário é o importador de registo e deve estar em conformidade com todas as leis e regulamentos do país de destino.
  • Nem todos os produtos listados na Ubuy estão à venda, uma vez que a Ubuy é um motor de busca global. Os produtos estão sujeitos a leis de exportação/comércio.